An assumed model for adsorbed BSA molecules based on critical oncentration and surface coverage

(a) Schematic drawing of the time evolution of the adsorbed cake layer model at low surface coverage.

(b) hypothetical model depicting the conformational change of adsorbed layer above critical concentration.

(c) schematic drawing of the time evolution of the adsorbed cake layer model above critical concentration.

 

Typical over-stack spectrums for AII area determination at different membrane depth from FTIR mapping

 

蛋白质吸附前的AFM图

 

蛋白质吸附后的AFM图

 

蛋白质脱附后的AFM图